anti plac8 (Proteintech)
Structured Review
Anti Plac8, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+plac8/PLAC8+Antibody/pmc12696950-67-65-66
Average 93 stars, based on 15 article reviews
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Incubation:Article Title: PLAC8 attenuates pulmonary fibrosis and inhibits apoptosis of alveolar epithelial cells via facilitating autophagy Article Snippet: The protein gels were transferred to PVDF membranes (IPVH00010, Millipore). .. The membranes were incubated with Western Blocking Buffer (SW3010, Solarbio) for 1 h followed by incubation with primary antibodies: anti-SPC (10774-1-AP), Article Title: Propagation of monocyte exhaustion memory and underlying mechanisms. Article Snippet: .. The membranes were then blocked with PBST buffer containing 5% non-fat milk (Bio-Rad, #170–6404) for 1 h, followed by overnight incubation at 4 °C with primary antibodies, including anti-STAT1 (Cell Signaling, #9172), anti-STAT3 (Cell Signaling, #12640), anti-Akt (Cell Signaling, #9272), anti-PGC1α/β (Invitrogen, #A5-32563), anti-S6K(P70) (Proteintech, #4485-1-AP), anti-RICTOR (Proteintech, #66867-2-Ig), anti-Src (Cell Signaling, #2110), anti-phospho-Src (Cell Signaling, #2105), anti-CREB (Cell Signaling, #9197), anti-phospho-CREB (Cell Signaling, #9198), Article Title: Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression Article Snippet: To detect PLAC8 in SW480 cells undergoing mitosis, 1 × 10 5 cells were grown on four-well cell culture slides (SPL Life Sciences, Gyeonggi-do, Korea), rinsed with phosphate-buffered saline (PBS), fixed in 4% paraformaldehyde for 10 min and permeabilized using 0.1% Triton X-100 in PBS for 35 min. .. Cells were then blocked in a blocking solution (1.5% horse serum in PBS) for 30 min, incubated with Article Title: Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression. Article Snippet: To detect PLAC8 in SW480 cells undergoing mitosis, 1 105 cells were grown on four-well cell culture slides (SPL Life Sciences, Gyeonggi-do, Korea), rinsed with phosphate-buffered saline (PBS), fixed in 4% paraformaldehyde for 10 min and permeabilized using 0.1% Triton X-100 in PBS for 35 min. .. Cells were then blocked in a blocking solution (1.5% horse serum in PBS) for 30 min, incubated with Article Title: Propagation of monocyte exhaustion memory and underlying mechanisms Article Snippet: .. The membranes were then blocked with PBST buffer containing 5% non-fat milk (Bio-Rad, #170–6404) for 1 h, followed by overnight incubation at 4 °C with primary antibodies, including anti-STAT1 (Cell Signaling, #9172), anti-STAT3 (Cell Signaling, #12640), anti-Akt (Cell Signaling, #9272), anti-PGC1α/β (Invitrogen, #A5-32563), anti-S6K(P70) (Proteintech, #4485-1-AP), anti-RICTOR (Proteintech, #66867-2-Ig), anti-Src (Cell Signaling, #2110), anti-phospho-Src (Cell Signaling, #2105), anti-CREB (Cell Signaling, #9197), anti-phospho-CREB (Cell Signaling, #9198), Article Title: Alleviation of monocyte exhaustion by BCG derivative mycolic acid Article Snippet: .. Post block, membranes were incubated with anti-Src (Cell Signaling), anti-phospho-Src (Cell Signaling), anti-Syk (Cell Signaling), anti-STAT1 (Cell Signaling), anti-phospho-STAT1 (Cell Signaling), anti-TAX1BP1 (Proteintech), Western Blot:Article Title: PLAC8 attenuates pulmonary fibrosis and inhibits apoptosis of alveolar epithelial cells via facilitating autophagy Article Snippet: The protein gels were transferred to PVDF membranes (IPVH00010, Millipore). .. The membranes were incubated with Western Blocking Buffer (SW3010, Solarbio) for 1 h followed by incubation with primary antibodies: anti-SPC (10774-1-AP), Article Title: Aberrant PLAC8 expression characterizes glioblastoma with temozolomide resistance and an immunosuppressive microenvironment. Article Snippet: Glioblastoma (GBM), Isocitrate Dehydrogenase-wildtype (IDH-WT) represents the most prevalent and clinically aggressive subtype of adult diffuse gliomas, typically associated with poor prognosis.. Temozolomide (TMZ) remains the first-line chemotherapeutic agent for GBM; however, the emergence of TMZ resistance represents a major therapeutic obstacle in clinical practice.. This study identifies placenta-specific 8 (PLAC8) as a novel mediator of TMZ resistance in IDH-WT GBM. Blocking Assay:Article Title: PLAC8 attenuates pulmonary fibrosis and inhibits apoptosis of alveolar epithelial cells via facilitating autophagy Article Snippet: The protein gels were transferred to PVDF membranes (IPVH00010, Millipore). .. The membranes were incubated with Western Blocking Buffer (SW3010, Solarbio) for 1 h followed by incubation with primary antibodies: anti-SPC (10774-1-AP), Article Title: Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression Article Snippet: To detect PLAC8 in SW480 cells undergoing mitosis, 1 × 10 5 cells were grown on four-well cell culture slides (SPL Life Sciences, Gyeonggi-do, Korea), rinsed with phosphate-buffered saline (PBS), fixed in 4% paraformaldehyde for 10 min and permeabilized using 0.1% Triton X-100 in PBS for 35 min. .. Cells were then blocked in a blocking solution (1.5% horse serum in PBS) for 30 min, incubated with Article Title: Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression. Article Snippet: To detect PLAC8 in SW480 cells undergoing mitosis, 1 105 cells were grown on four-well cell culture slides (SPL Life Sciences, Gyeonggi-do, Korea), rinsed with phosphate-buffered saline (PBS), fixed in 4% paraformaldehyde for 10 min and permeabilized using 0.1% Triton X-100 in PBS for 35 min. .. Cells were then blocked in a blocking solution (1.5% horse serum in PBS) for 30 min, incubated with Article Title: Alleviation of monocyte exhaustion by BCG derivative mycolic acid Article Snippet: .. Post block, membranes were incubated with anti-Src (Cell Signaling), anti-phospho-Src (Cell Signaling), anti-Syk (Cell Signaling), anti-STAT1 (Cell Signaling), anti-phospho-STAT1 (Cell Signaling), anti-TAX1BP1 (Proteintech), Cell-Signaling:Article Title: Alleviation of monocyte exhaustion by BCG derivative mycolic acid Article Snippet: .. Post block, membranes were incubated with anti-Src (Cell Signaling), anti-phospho-Src (Cell Signaling), anti-Syk (Cell Signaling), anti-STAT1 (Cell Signaling), anti-phospho-STAT1 (Cell Signaling), anti-TAX1BP1 (Proteintech), |

